Journal: BMC Oral Health
Article Title: Low-level laser therapy prevents medication-related osteonecrosis of the jaw-like lesions via IL-1RA-mediated primary gingival wound healing
doi: 10.1186/s12903-022-02678-1
Figure Lengend Snippet: Application of IL-1RA NAb impairs the capacity of LLLT to promote gingival wound healing. A The study's experimental protocols are depicted in this diagram. B Representative intraoral photos. (Ctrl: non-drug treatment, but treated with negative control antibodies group; Zol: treated with Zol and negative control antibodies group; Zol + LLLT: treated with Zol, LLLT, and negative control antibodies group; Zol + LLLT + IL-1RA: treated with Zol, LLLT, and IL-1RA NAb group). Green dotted lines represent unhealed gingiva. C MicroCT reconstructed 3D images of tooth sockets. Green dotted lines represent tooth sockets. D , E Quantification of BMD and BV/TV in each group. F H&E staining shows tooth extraction sockets-wound healing in each group. Black dotted boxes represent magnified boxed regions. Green dotted lines represent unhealed gingiva. Scale bar = 200 μm (upper), scale bar = 100 μm (lower). G , H Images of IL-1β and TNF-α IHC staining of the mouse gingival wound. Scale bar = 100 μm. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)
Article Snippet: The area of interest was chosen, and bone mineral density (BMD) and bone volume/total volume (BV/TV) were quantified by three-dimensional (3D) bone morphometric software (SIEMENS, Munich, Germany).
Techniques: Negative Control, Staining, Immunohistochemistry